Exploring the Microbiome of Healthy and Diseased Peri-Implant Sites Using Illumina Sequencing

Aim To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome. Materials and Methods Submucosal biofilms from 32 H and 35 P sites were analyzed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using Principal Coordinate Analysis (PCoA), t-tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined. Results PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p\u3c0.05). Porphyromonas and Treponema were more abundant in P and while Rothia and Neisseria were higher in H (p\u3c0.05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola and P. gingivalis levels were higher in P, as well as F. alocis, F fastidiosum and T. maltophilum (p\u3c0.05). Conclusion The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harboring traditional and new pathogens. The core peri-implant microbiome harbors taxa from genera often associated with periodontal inflammation

Les proteines, molecules modulaires

De nombreuses proteines sont constituees d'un petit nombre de domaines independants. On decouvre comment ces domaines se sont dissemines parmi les proteines et parmi les especes vivantes au cours de l'Evolution